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KMID : 1134819990280030663
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Journal of the Korean Society of Food Science and Nutrition
1999 Volume.28 No. 3 p.663 ~ p.669
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Effects of Vitamin E on the Microstructural Changes of Renal Tissue in Streptozotocin - Induced Diabetic Rats
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Kwag Oh-Gye
Im Jung-Gyo
Rhee Soon-Jae
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Abstract
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The purpose of this study was to investigate the effects of vitamin E on the histochemical change of kidney tissue in diabetic rats. Sprague-Dawley male rats weighing 100±10g were randomly assigned to one normal and three STZ-induced diabetic groups, which were subdivided into vitamin E free diet(DM-0E group), 40㎎ vitamin E per ㎏ diet(DM-40E group) and 400㎎ vitamin E per ㎏ diet(DM-400E group). Vitamin E level of normal group was 40㎎ per ㎏ diet. Diabetes was experimentally induced by intravenous injection of 55㎎/㎏ of body weight of streptozotocin(STZ) in citrate buffer(pH 4.3) after 4 weeks feeding of experimental diets. Animals were sacrificed at the 6th day of diabetic states. The contents of thiobarbituric acid(TBARS) in kidney were increased 119%, 84% and 33% in DM-0E, DM-40E and DM-400E groups, respectively, compared to normal group. That of DM-400E group was decreased 39% compared to DM-0E group. Content of β₂-microglobulin in urine in DM-0E, DM-40E, and DM-400E groups were increased by 248%, 181%, and 164%, respectively, compared to normal group. The diabetic groups showed the regressive lesion such as renal tubule, intumescence of epithelial cell, vacuolization. The results of the observation through electronic microscope showed the mitochondria shape of proximal tubule epithelial cell, irregular array, increase of ribosome, and irregular arrangement of small villosity, etc. These types of changes appeared severer in DM-0E group than in DM-400E group. These results indicate that the TBARS productions on kdney in STZ-induced diabetic rats were increased, consequently those leaded to damage of renal tubule and minuteness structure. But a large quantity vitimin E supplementation was suppressed in TBARS production and improved in peroxidative damage of renal tissue so that relieved degenerative changes of renal tubule epithelial cell.
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KEYWORD
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vitamin E, diabetic rat kidney, microstructural change
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